We found a significant reduction in Lysotracker staining in both infected (mCherry + /GFP+) and exposed bystander (mCherry + /GFP-) macrophages from larvae treated with the Kmo inhibitor compared to vehicle control (Figs 3D, 3E, S7A), demonstrating a global reduction in lysosome acidification regardless of infection status. This evidence concerns the gene KMO and infection.