CD28 and neoplasm: Dot plot analysis of differentiated gene expression confirmed a high proportion of CD8+ T cells in tumor following dual LLL–nanoSTING@Mn treatment, with elevated expressions of genes associated with mitochondrial activation (Cd28, Cox16),[44] memory function (Cd44, Il7r, Il2rb, Cd27, Eomes),[45] and cytotoxic effector function (Nfatc3, Tia1, Klrd1) (Figure 3D).[45, 46, 47] By contrast, treatment with nanoSTING@Mn alone did not enhance the expression of these genes as compared to the controls.