The rHSP83 antigen, although not yet analyzed, has been investigated in serum samples with cutaneous leishmaniasis and visceral leishmaniasis, with a specificity of 100% observed when tested against serum samples from individuals with Chagas disease (Celeste et al., 2004), unlike the results observed for CVL, in which moderate cross-reactivity was found with sera from dogs infected with E. canis. The gene discussed is EZR; the disease is visceral leishmaniasis.