To gain insight into KCa3.1 interactions and mechanism in renal epithelial fluid secretion during cyst enlargement, we next evaluated cyst enlargement in Pkd1–/– metanephroi treated with senicapoc alone or in combination with inhibitors of apical Cl– channels TMEM16A/Ano1 and CFTR, previously shown to block cyst growth (51, 52). This evidence concerns the gene CFTR and cyst.