The AUC analysis at day 17 demonstrated a significant reduction in tumor size with dual anti–PD-1/anti-MIF treatment as well as anti–PD-1 and anti-MIF monotherapy when compared with the ctrl group (P < 0.001, P < 0.001, and P = 0.0176, respectively) (Figure 1H).To evaluate the presence of tumor immune memory in the YUMMER1.7 model, we rechallenged animals that had a complete rejection of tumor with a larger tumor load and observed the animals without treatment (Supplemental Figure 1A; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.191539DS1). This evidence concerns the gene PDCD1 and neoplasm.