EcoHIV NL4-3-eGFP does not use CD4, CXCR4, CCR5 receptors that are conventionally required for HIV infection, or other viral receptors such as DC-SIGN or CLEC-2 for entry into cells and is highly specific to cells expressing mCAT-1 (Alfar et al. 2024). Here, CD4 is linked to HIV infectious disease.