RFWD3 and neoplasm: First, C57BL/6 mice were subcutaneously transplanted with control or RFWD3 knockdown murine Lewis Lung Carcinoma (LLC) cells that are insensitive to immune checkpoint inhibitors (ICI),[25] and we found that knockdown of RFWD3 (Figure 4A) significantly inhibited tumor growth (Figure 4B) and reduced tumor weight (Figure 4C).