To investigate whether the conditioned media from Rfwd3‐knockdown tumor cells can activate immune cells, we co‐cultured LLC cells and RAW‐Lucia ISG cells that were generated from the murine RAW 264.7 macrophage cell line by stable integration of an interferon regulatory factor (IRF)‐inducible Lucia luciferase reporter construct. This evidence concerns the gene RFWD3 and neoplasm.