TLR4 and infection: In contrast, overexpressing Spz (Da>Spz*) and Toll (Da>Tl10B) in Drosophila significantly increased fly antifungal abilities (p < 0.001) (Figure 5C), which is in line with previous findings.[40, 41] Interestingly, Da>Tl10B females were equally susceptible to topical infection with the WT, ΔETS1, ΔETS6 and ΔETS1.6 strains of M. robertsii (Figure 5D).