RT‐qPCR results demonstrated that neutrophils exposed to conditioned medium from CXCL2 overexpressing HCC cells showed significantly higher expression of iNOS and other N1 markers, but lower expression of CD206 and other N2 markers, compared to neutrophils exposed to the conditioned medium from the NC group (Figure 2H,I). The gene discussed is CXCL2; the disease is hepatocellular carcinoma.