Based on the high expression of specific marker genes for each cell type, we identified seven major cell populations (Figure 1B; Figure S1A, Supporting Information).These included B cells (CD79A, MS4A1, and CD20), dendritic cells (ITGAX and CD40), myeloid cells (CD209, CD68, CD14, and CD163), NK and T cells (CD3D, CD3E, and GNLY), cancer cells (CDKN2A, EPCAM, and KRT18), endothelial cells (PECAM1, CD34, and VWF), and fibroblasts (DCN, THY1, and ACTA2) (Figure 1C).[24, 25] The spatial distribution of these cell types is shown in Figure S1B (Supporting Information). The gene discussed is CD40; the disease is cancer.