The <sup>125</sup>I uptake assay confirmed stimulation of functional NIS expression in SMAD-NIS-MSCs through TGF-β1 released from BRAF<sup>V600E</sup>-mutant PTC cell lines (BCPAP, K1) from co-culture or by incubation with tumor-conditioned medium. This evidence concerns the gene TGFB1 and neoplasm.