In mice, it was shown that chronic ethanol feeding influenced eight canonical pathways in the liver, including tight junction signaling, pulmonary fibrosis idiopathic signaling pathway, integrin-linked kinase signaling, hepatic fibrosis signaling, Wnt/β-catenin signaling, clear signaling, TGF-β1 signaling, production of nitric oxide and ROS in macrophages, phosphatase and tensin homolog (PTEN) signaling, and inhibitor of differentiation.69 Here, PTEN is linked to pulmonary fibrosis.