In this study, we showed that knockdown of YAP mitigated HMGB1-tigerred PASMC glycolysis, and inhibition of YAP by VER suppressed glycolysis to blunt pulmonary vascular remodeling, suggesting the crucial role of YAP in PASMC glycolysis in PAH. The gene discussed is HMGB1; the disease is pulmonary arterial hypertension.