Given that the limbic regions are primary sites of TDP-43 pathology in AD patients (Amador-Ortiz et al, 2007; Arai et al, 2009; Josephs et al, 2015; Latimer and Liachko, 2021; Meneses et al, 2021), we further performed double fluorescence immunostaining in the hippocampus tissue. This evidence concerns the gene TARDBP and Alzheimer disease.