To investigate the disease relevance of Tau/TDP-43 interaction, we extracted sarkosyl-insoluble material from the frontal cortex of patients diagnosed as AD− (containing Tau pathology, but lacking TDP-43 pathology), AD+ (Tau+TDP-43 pathology, consistent with limbic predominant age-related TDP-43 encephalopathy neuropathological changes (LATE-NC) (Nelson et al, 2019)), FTLD-Tau, FTLD-TDP Type A or non-neurodegeneration controls (non-ND), and from the cingulate cortex of PD patients (Table EV1), following the established SarkoSpin procedure (Laferrière et al, 2019). Here, MAPT is linked to neurodegenerative disease.