APOE and Alzheimer disease: Interestingly, comparing miBrains constructed with cells derived from an isogenic iPSC line CRISPR-edited to APOE4/4 AD risk, BMEC monolayers seeded with these APOE4/4 miBrains had decreased barrier function compared to APOE3/3 miBrains as assessed via TEER on a transwell system (SI Appendix, Fig. S16C).