We further employed GSEA to explore the functional pathways enriched by genes, revealing that DNAJB9 was significantly enriched in the mTOR signaling pathway (ES = 0.639, NES = 1.49, p = 0.0000), thyroid cancer (ES = 0.714, NES = 1.65, p = 0.0176), and folate biosynthesis (ES = 0.852, NES = 1.60, p = 0.0228)(Fig 5H). The gene discussed is DNAJB9; the disease is thyroid gland carcinoma.