BDNF and Anxiety: This may be associated with electroacupuncture used in this study, which enhances the expression of hippocampal brain-derived neurotrophic factor (BDNF) and reshapes the functional connectivity of the prefrontal-limbic system through quantifiable electrical stimulation parameters and sustained duration, forming a multi-level anti-anxiety mechanism by promoting neuroregeneration and plasticity and restoring the balance of emotional regulation circuits, thereby demonstrating superior rapidity and sustainability in clinical anti-anxiety effects compared with traditional acupuncture (37–40).