To investigate if RNF4 depletion sensitizes AML cells to genotoxic stress, we performed an siRNA-mediated knockdown (KD) of RNF4 in OCI-AML2 cells (Fig. EV1E), followed by treatment with the DNA damage compounds decitabine and aphidicolin (Fig. 1E). Here, RNF4 is linked to acute myeloid leukemia.