NPM1 and acute myeloid leukemia: The WT1 gene, in addition, is overexpressed in 75–100% of adult AML patients, and its high expression is associated with different molecular alterations such as FMS-like tyrosine kinase-3 internal tandem duplication (FLT3-ITD), nucleophosmin 1 (NPM1) mutation, CBFb: MYH11 gene fusion, as well as KMT2A rearrangement, while a low expression is observed in RUNX1:RUNX1T1 AML.