APC and colorectal cancer: Comparing the predicted distribution with independent colorectal cancer cohorts (Fig. 3F; Supplementary Fig. S6), we found that the proposed model explains most of the variability in the distribution of APC mutations observed in MSS colorectal cancers (R2 = 0.942 for 100kGP, R2 = 0.963 for cBioPortal, and R2 = 0.913 for the cohort in Christie and colleagues, ref. 21) and accurately predicts the distribution of mutations by APC region (Fig. 3G).