Next, the DM model was established using streptozotocin (45 mg/kg) for 2 consecutive injections, and DMED rats were screened by apomorphine and mating test at weeks 2, 4, 6, and 8, and then, we intervened for a fortnight using Yimusake (Y) and NLRP3 inhibitor (MCC950) drugs, and the penile tissues were taken for the subsequent analyses. This evidence concerns the gene NLRP3 and diabetes mellitus.