We assigned each retina a CE score ranging from 0 to 5 using a grading scale described in Supplemental Figure 1; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.190227DS1 This analysis revealed that the vast majority of Tspan12-KODBM mice displayed CE, whereas CE in Tspan12-ECKO mice was sporadic and milder (Figure 1D). This evidence concerns the gene TSPAN12 and cholesteryl ester measurement.