Both CH and BC demonstrated a significantly rapid reduction (p < 0.0001) in cell viability compared to the controls after 48 h, resulting in 36.72 ± 9.55% and 10.70 ± 3.22% viable cells, respectively. On days 7, 14, and 21, the effect of CH remained consistent, followed by a rapid increase on day 28, resulting in 133.97 ± 1.46% viable cells. This evidence concerns the gene C4B and breast cancer.