To shed light on whether KDM6A and KDM6B demethylases are involved in H3K27me3 to H3K27ac switches in enhancers regulated by EWSR1::FLI1, we carried out ChIP-seq for KDM6A, KDM6B, and EWSR1::FLI1 in the Ewing sarcoma cell line A-673. Here, KDM6B is linked to Ewing sarcoma.