Studies in patients with Alzheimer's disease and animal models suggest that APOE can alter the microglial transcriptome, affecting immune responses, lipid metabolism, IFN signaling, and other functions.[35, 36] Our pseudotime analysis indicates that under epileptic conditions, APOE is the main driver of microglial differentiation. This evidence concerns the gene IFNA1 and early-onset autosomal dominant Alzheimer disease.