Integrating the VIP scores of key metabolites with the results from KEGG enrichment analysis, six key metabolites significantly regulated by APOE and closely associated with epilepsy progression were identified: cardiolipin (CL, 17:1/18:0/18:0/18:1), PS (18:0/22:6, 18:1/18:2), phosphatidylglycerol (PG, 22:5/20:4), phosphatidic acid (PA, 18:0/20:4), and monolysocardiolipin (MLCL(10:0/18:0/22:6)) (Figure 8I). This evidence concerns the gene APOE and epilepsy.