PECAM1 and neoplasm: First, we separated tumor cells (CKAE1/3+; n = 266,172) from endothelial cells (CD31+, CKAE1/3−; n = 1,349), immune cells (CKAE1/3−, CD45+, or CD138+; n = 50,432), and CAFs (CKAE1/3/CD45/CD31/CD138−; n = 90,986).