To examine the possible physiological function of Tls, we deleted tls gene in E. coli B2 strain S13, a septicemia isolate that was previously used to investigate the regulation of motility in pathogenic E. coli (Laganenka et al, 2020) and is similar to the prototypic uropathogenic E. coli UTI89 (Fig. 1C; Reagents and Tools Table and Appendix Table S1). Here, FUS is linked to Sepsis.