In conclusion, our study demonstrates that Gln strengthens macrophage phagocytosis and alleviates immunosuppression in sepsis through a dual GFAT-DRP1 mechanism co-ordinating mitochondrial dynamics and calcium signaling, highlighting the GFAT–DRP1–calcium axis as a potential therapeutic target for treating sepsis-induced immunosuppression. This evidence concerns the gene DNM1L and Sepsis.