To evaluate changes in autophagy within tumor tissues, immunofluorescence and immunohistochemistry staining of LC3, a classical autophagy marker, showed significantly higher expression levels in the LSPs group, which were markedly diminished following laser ablation or autophagy inhibition, confirming that autophagy was the main mode of cell death after 14 days of LSPs treatment (Figure 5E,F). The gene discussed is MAP1LC3A; the disease is neoplasm.