These results confirm our previous findings of increased cholesterol synthesis in PMS iNSCs.18 Leveraging published GWAS studies, we identified genes associated with MS progression and pathology, such as leukocyte activation and differentiation, STAT signaling, and IFN production.60–62 We also identified IRF5, a known gene variant in MS,63 to be hypomethylated at the promoter-TSS region in both PMS fibroblasts and iNSCs (Figure 2H). Here, IRF5 is linked to myeloid sarcoma.