S100A4 and breast neoplasm: Each sample includes five channels: S100A4-Opal 520, CK-Opal 570, PDPN-Opal 650, 4′,6-diamidino-2-phenylindole (DAPI) and bright field with 3,506 × 3,506 pixels along XY (see ‘Multichannel breast tumor data’ section in Methods).