NDST1 and obesity due to melanocortin 4 receptor deficiency: To study the impact of changes in HS composition on obesity and T2D development we inactivated the sulfotransferase, Ndst1, in adipocytes (Ndst1flox/floxAdipoq-Cre+ [Ndst1 AKO]) and put the mice on a HFD (Figure 1A & Supplementary Fig. 1b).