Our data show that prolonged treatment with 2DG is well tolerated by hippocampal neurons in vitro, increases REST/NRSF expression and its translocation to the nucleus and induces a reduction in the NADH/NAD+ ratio, as previously observed both in response to 2DG (Shen et al., 2017) and hypoglycaemia (Lee et al., 2024). This evidence concerns the gene REST and Hypoglycemia.