This study aims to investigate the impact of PD-L1 on the signaling pathways in EGFR-mutant NSCLC.<h4>Methods</h4>The regulatory role of PD-L1 was investigated through in vitro manipulation of PD-L1 expression across several EGFR-mutant cell lines, followed by analysis via human receptor tyrosine kinase (RTK) array, Western blotting, protein tyrosine phosphatase (PTPs) activity assays, and mRNA expression profiling. Here, CD274 is linked to non-small cell lung carcinoma.