This study aims to investigate the impact of PD-L1 on the signaling pathways in EGFR-mutant NSCLC.<h4>Methods</h4>The regulatory role of PD-L1 was investigated through in vitro manipulation of PD-L1 expression across several EGFR-mutant cell lines, followed by analysis via human receptor tyrosine kinase (RTK) array, Western blotting, protein tyrosine phosphatase (PTPs) activity assays, and mRNA expression profiling. The gene discussed is PTS; the disease is non-small cell lung carcinoma.