DEFA5 and inflammatory bowel disease: Affinity-purified recombinant 1A8 and 4F5 antibodies, expressed in HEK293 cells, showed binding affinities to recombinant DEFA5 (indirect ELISA) and DEFA5 in IBD biopsy samples (sandwich ELISA) comparable to the parental mouse monoclonal antibodies, confirming the sequencing data (data not shown)