LDLR and obesity due to melanocortin 4 receptor deficiency: Ldlr–/– mice were subjected to a high-fat, high-cholesterol (HFHC) diet for 20 weeks (obese baseline, Ob), which, as expected, induced obesity, hypercholesterolemia (Supplemental Figure 1, A and B; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.193593DS1), and advanced atherosclerotic plaques.