When we tried to perform these experiments in a converse fashion using YAP1- and TAZ-KO models in our IDHwt glioma cultures, we were never able to establish more than partial/hemizygous KO in both GBM43 and 0923 using 4 different TAZ gRNAs at an MOI of 30, suggesting a unique difficulty for eliminating TAZ function in IDHwt glioma (not shown). The gene discussed is WWTR1; the disease is central nervous system cancer.