Based on negative binomial regression, groups of obesity saw a significant positive correlation between CAL/PD and ISM1, MMP-8, asprosin in GCF, as well as BMI.<h4>Conclusion</h4>In conclusion, circulating levels of ISM1 and MMP-8 were significantly elevated in patients with obesity and positively correlated with BMI. Here, ISM1 is linked to obesity due to melanocortin 4 receptor deficiency.