To confirm the physiological relevance of this interaction, we showed that endogenous OTUD4 and TAK1 associate in H1299 NSCLC cells (Fig. 2, G and H) and significantly colocalize in the cytoplasm (Pearson's correlation coefficient [PCC] = 0.66 ± 0.15; Fig. 2I). Here, MAP3K7 is linked to non-small cell lung carcinoma.