To test the hypothesis that in vivo detection of potential TLR7 agonist-mediated virus induction is dependent upon the size or nature of the established viral reservoirs, which may practically depend upon the timing of cART initiation and the sensitivity of the virologic assays employed, in the present study, we treated SIV-infected rhesus macaques with cART starting 65 days post-infection to better match the conditions of the Lim et al. study. Here, TLR7 is linked to infection.