Single-nucleotide polymorphisms associated with HK1 were selected as instrumental variables, and MR analyses were performed using inverse-variance weighted as the primary study method, and MR-Egger, weighted median, simple mode, and weighted mode as complementary methods in order to assess whether HK1 is a protective factor for NSCLC, and to provide further evidence that lactic acid is a risk factor for NSCLC. This evidence concerns the gene HK1 and non-small cell lung carcinoma.