CASP8 and cancer: To confirm that Caspase-8 may sustain NFE2L2 gene expression, NRF2 mRNA and protein levels were evaluated in GBM cell lines and in patient derived cancer stem cells grown as neurospheres stably genetically silenced (shC8) or not (shCTR) for Caspase-8 expression (Fig. 1B–G) [8], as well as in U87-MG cells transiently silenced (siC8) or not (siSCR) for Caspase-8 expression (Supplementary Fig. S1D).