APC and colorectal carcinoma: Analogous to the initial evaluation of the AKP mice by Tang and colleagues (and earlier studies of models targeting only APC) using genotyping and/or IHC (45, 114–116), we initially sought to verify biallelic Apc inactivation (through somatic alteration of the second non-floxed Apc allele) in AK and AKP tumors with MiMouse using the Trp53 flox CN as a reference, as these colorectal carcinoma models conditionally lose either both Trp53 alleles (flox/flox) or only one Trp53 allele [with the other allele having an oncogenic mutation (flox/R270H)].