Although MiMouse did not profile Smad2 or Dcc, in support of our hypothesis, 50% (14 of 28) of colorectal adenocarcinoma samples had single-copy Smad4 loss [vs. one of 10 (10%) of adenomas], supporting the fidelity of the AKP model to Smad4 single-copy loss, although through a different mechanism than in human tumors due to synteny. Here, DCC is linked to adenoma.