CCNB2 and benign prostatic hyperplasia: Then, we analyzed the expression of the CCNB2 gene in PCa and benign prostatic tissues using western blot and qPCR: Compared to RWPE-1 (benign prostatic hyperplasia tissue), the expression levels of CCNB2 in PC3 and DU145 were higher in PCa tissues, while C4-2 exhibited lower expression levels in tumors (Figure 7B, E).