In this study, an in-depth investigation of CGB5 in pan-cancer was conducted through multiple public databases, including The Cancer Genome Atlas (TCGA), Human Protein Atlas (HPA), UALCAN, cBioPortal Platform, Gene Set Cancer Analysis (GSCA), Kaplan–Meier Plotter, TIMER, TISIDB, SangerBox Website, and Metascape database. This evidence concerns the gene CGB5 and cancer.