Moreover, 0.3 mg/kg dose led to changes in multiple pathways, including biosynthesis of unsaturated fatty acids, insulin signaling pathway, AMPK signaling pathway, insulin resistance, fatty acid degradation, retinol metabolism, fatty acid metabolism, fatty acid elongation, glucagon signaling pathway, type II diabetes mellitus, and PPAR signaling pathway (Figure 3B). The gene discussed is GCG; the disease is Insulin resistance.