Trajectory analysis identified three differentiation lineages from Tpex1 (Figure S7D), two of which led to dysfunctional tumor-reactive CD8+ T cells with Tpex2 as an intermediate: Tpex1 → Tpex2 → Tex (lineage 1, Figure S7E) and Tpex1 → Tpex2 → HSP+ cells (lineage 2, Figure S7F). This evidence concerns the gene CD8A and neoplasm.