Treatment with bioactive compounds such as withaferin-A (WA) and 5-fluorouracil (5-FU) or acetate extract of cichorium (EAEC) in HCT-116, SW-480, and HT-29 cells has shown its anti-cancer effects by activating the PERK arm, leading to apoptosis and autophagy [16, 21]. The gene discussed is EIF2AK3; the disease is cancer.