H2BK5ac, H2BK20ac, histone H3 lysine 18 acetylation (H3K18ac), histone H3 lysine 27 acetylation (H3K27ac) and p300 chromatin immunoprecipitation sequencing (ChIP-seq) confirmed that the majority of H2BK5ac (82.3%) and H2BK20ac (76%) histone marks overlapped with p300 peaks in prostate cancer cells, while a smaller fraction of H3K18ac (46.7%) and H3K27ac (46.3%) sites overlapped with p300 peaks, supporting the dependency of H2BNTac on p300 (Extended Data Fig. 1h,i). This evidence concerns the gene EP300 and prostate cancer.