Out Of 11 unique PTEN variants identified, two corresponded to novel variants in astrocytoma patients (g.chr10:87925553 A > G (AF: 43.6%; SIFT: deleterious; Polyphen-2: probably damaging) and g.chr10:87933224_87933225insG (AF: 43.7% SIFT indel: damaging). The gene discussed is PTEN; the disease is astrocytoma (excluding glioblastoma).