Thus, novel PTEN (frameshift deletion g.chr17:7674966delC) and TP53 variants (missense mutation g.chr10:87925553 A > G and frameshift insertion g.chr10:87933224_87933225insG) with predicted damaging effects were identified in our study, expanding the known mutations in these genes in patients with astrocytomas. Here, PTEN is linked to astrocytoma (excluding glioblastoma).